电离辐射对大鼠十二指肠内亮脑啡肽神经及亮脑啡肽含量的影响

Effect of Ionizing Radiation on Leucin enkephalin in Rat Duodenum

作者应用免疫细胞化学和放射免疫分析(RIA)等方法观察4Gy和25Gy ^(60)Co7射线照射后24,48和72h大鼠十二指肠亮脑啡肽(L-ENK)的分布及含量的变化。光镜下见各受照组L-ENK样免疫反应性(L-ENK-LI)神经纤维的分布无明显改变,25Gy照射后电镜下可见肌间神经节细胞胞突肿胀,微丝、微管破坏,从24h至72h有逐渐加重趋势.RIA结果显示,4Gy照射后48h及25Gy照射后24hL-ENK含量分别为14.98±0.93ng/g和14.90±1.38ng/g正常对照组L-ENK含量为10.82±0.98ng/g与正常对照组相比,4Gy照后48h和25Gy照后24h LENK含量明显升高(P<0.05)L-ENK含量的升高可能参与了受照后肠道运动功能紊乱等过程.

The distribution and content of Leucin-enkephalin (L-ENK) in rat duodenum were studied by immunocytochernistry and radioimmunoassay 24, 48 and 72 hours after exposure to 4 Gy and 25 Gy60 Co γ- ray. No obvious changes of distribution pattern of L-ENK-like immunorcacu'vc (L-ENKLI ) fibres were observed under a light microscope in all irradiation groups. The progressive swelling of nervous process was found in the myenteric ganglia under an electron microscope 24 to 72 hours after 25 Gy irradiation. The L-ENK content in duodenum was 14.98 ± 0.93 ng/g and 14.90 ± 1.38 ng/g of wet. tissue with RIA 48 h after exposure to 4 Gy and 24 h after exposure to 25 Gy γ -ray respectively. It was significantly higher than that in the normal control group. The increase of L-ENK content probably involved in the disturbance of intestinal motility after irradiation.