胎肝细胞分泌的GM-CSF协同刺激因子的初步研究

A new hematoietic stimulating activity produced by fetal liver cells. CSF synergistic activity

本文观察到体外培养12h收获的胎肝细胞条件培养液(FLCM)在外源性集落刺激因子(GM-CFU)存在时,能明显促进粒-单系祖细胞(CFU-GM)的生长和提高骨髓细胞S期DNA的合成。结果提示FLCM中存在某种能与GM-CSF协同的造血刺激因子。

Human fetal liver cells were cultured in vitro for 12 hours and the supernatant ( FLCM, fetal liver cell conditioned medium ) was collected. The effects of FLCM on granulopoiesis were studied. The results showed that when used with GM-CSF, FLCM could significantly stimulate the proliferation of CFU-GM, and increase 3H-TdR incorparation of bone marrow cells, suggesting that there is a GM-CSF synergistic stimulating factor in FLCM.