慢性乙型肝炎病毒感染者血清前S_2蛋白和聚合人血清白蛋白结合活性的对比研究

A comparative study on serum preS2 and polymerized human albumin binding activity in patients with chronic hepatitis B virus infection

以合成的前S2肽免疫兔获得抗血清。分别以抗前S2抗体和聚合人血清白蛋白作试剂,用固相放免法检测前S2和聚合人血清白蛋白结合活性。在甲型肝炎和HBsAg(-)的各类慢性肝病病人中均未检出。在经肝活检诊断的慢性乙型活动性肝炎,两者的检出率在病情增重期显著高于缓解期,并高于肝组织正常的无症状HBV携带者。在HBeAg(+)组中两者的检出率均显著高于抗HBe(+)组。在上述检测中,聚合人血清白蛋白结合活性与前S2并不相同,后者的检出率仅为前者的56.7％。

Antiserum against preS2 peptide was raised with a synthetic polypeptide from the rabbits. The anti-preS2 antibody and polymerized human serum albumin ( pHSA ) were used as reagents in a radioimmunoassay to detect preS2 and pHSA binding activity respectively. Both were absent among patients with hepatitis A and HBsAg negative chronic liver diseases. In biopsy-proven chronic active hepatitis ( CAH ) B, prevalence of both tests were significantly higher at exacerbation than at remission of the disease, and so were in CAH than in chronic asymptomatic HBV carrier with normal histology. Besides, the prevalence were significantly higher in HBeAg positive group than in anti-HBe positive group. However, the pHSA binding activity and the preS2 were undoubtedly not the same, as the prevalence of the latter was only 56.7% of the former.