摘要
With designed specific primers based on Potato leafroll virus(PLRV) coat protein(CP) gene sequence,one PCR fragment(about 0.6 kb) was amplified by reverse transcription polymerase chain reaction(RT-PCR) using the total RNA as template extracted from virus infected potato plant collected in Zhaotong,Yunnan province.The fragment was cloned into pGEM-T easy vector and sequenced.The sequence was compared with that of homologous gene of other PLRV isolates.The results showed it had high homology with the other isolates(the highest homology reached 99.2% of nucleic acid).Based on CP amino acid sequence the phylogenic tree of PLRV was established and the isolates were clustered into many groups.
With designed specific primers based on Potato leafroll virus（PLRV） coat protein （CP） gene sequence, one PCR fragment （ about 0.6 kb） was amplified by reverse transcription polymerase chain reaction （RT-PCR） using the total RNA as template extracted from virus infected potato plant collected in Zhaotong, Yunnan province. The fragment was cloned into pGEM-T easy vector and sequenced. The sequence was compared with that of homologous gene of other PLRV isolates. The results showed it had high homology with the other isolates（ the highest homology reached 99.2% of nucleic acid）. Based on CP amino acid sequence the phylogenic tree of PLRV was established and the isolates were clustered into many groups.
出处
《植物病理学报》
CAS
CSCD
北大核心
2006年第5期473-476,共4页
Acta Phytopathologica Sinica
基金
云南省自然科学基金重点项目资助(2005C0012Z)
