康氏木霉降解麸皮的研究

Study on Wheat Bran Degraded by Trichoderma koningii

对实验室分离筛选的康氏木霉进行研究。研究和分析了滤纸酶活(FPA)测定法的最优酶解条件为:在60℃、pH4.5下酶解40min,酶与底物之比为1:2。康氏木霉的最优产酶条件为28℃下,培养4d,培养基pH为6.5,含水量为150%,接种量为5%。用同时去蛋白和淀粉的麸皮作为作用底物,麸皮的转化率可高达28.5%。

Trichoderma koningii which had been separated was studied in this paper. The conditions of the filter paper activity （FPA） test were studied and analyzed, which were showed as follows： enzymolysis for 40 minutes on the condition of 60℃ and pH4.5, proportion of cellulase to substrate was 1：2. The optimum fermentation conditions were showed as follows： the culture medium was pH6.5, water content was 150%, the amount of inoculation was 5%, temperature was 28℃, fermentation time was 4d. The transformation efficiency could reach 28.5% when the wheat bran had been removed protein and starch.