摘要
本文采用超声辅助氢氧化钠提取菜籽饼粕中的多糖(RSPS),优化了提取条件,并对其进行Sevage法脱蛋白,DEAE-SephadexA-25柱和SephadexG-100柱分离,得到四个级分多糖(RSPS1～4),进而初步研究了其理化性质,并对其中两个较纯级分的多糖采用HPLC法进行了单糖组成分析。
The polysaccharide on repseed meal was extracted with ultrasonic-NaOH in this paper, the condition was optimized, and the protein was removed by Sevage, RSPS 1-4 were obtained by purify RSPS by DEAE-Sephadex A-25 and Sephadex G- 100. The physical and chemical characteristic of RSPS 1 - 4 were analyzed and the monosaccharide content of RSPS-2 and RSPS-3 were analyzed by HPLC.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第10期345-349,共5页
Food Science
关键词
超声辅助碱提
菜籽多糖
分离纯化
理化性质分析
单糖组成
extraction with ultrasonic-NaOH
polysaccharide on rapseed meal
isolation and purification
physicalcochemical characteristic assay
monosaccharide component
