桔梗对高血糖小鼠短期血糖和胰岛素水平影响

Influence of Platycodon grandiflorum(Jacq.)A.DC in Blood Glucose and Insulin Levels of Diabetic Mice

目的:研究桔梗醇提物对高血糖ICR小鼠短期血糖和胰岛素水平影响。方法:采用链脲菌素尾静脉注射得到高血糖模型小鼠,分别以桔梗醇提物、格林苯脲或水进行灌胃,观察小鼠空腹血糖、糖耐量水平、外源胰岛素敏感性和内源胰岛素水平变化。结果:短期服用桔梗醇提物能缓解高血糖小鼠因葡萄糖刺激的血糖升高;与模型对照组比较,桔梗醇提物明显降低了高血糖小鼠血糖水平;桔梗醇提物可以增加外源胰岛素作用效果,但不能改变内源胰岛素的水平。

This study for the first time is to examine the hypoglycemic effects of Platycodon grandiflorum （Jacq.） A. DC in streptozotocin （STZ） -induced diabetic ICR mice. The effects ofP. grandiflorum ethanol extract （PGE） on blood glucose, exogenous insulin sensitivity and plasma insulin levels were investigated. In four doses of extracts from the root of P. grandiflorum, extract at dose of 100mg/kg significantly suppressed the rise in blood glucose after 30min in the acute glucose tolerance test. Furthermore, this dose was applied in the fellow experiments. A significant decrease in blood glucose levels was observed after treatment of PGE. A combination of PGE and glibenclamide produced a greater effect in blood glucose level than using ghbenclarnide or PGE alone. PGE enhanced and prolonged the hypoglycemic effects of exogenous insulin on diabetic mice. Plasma insulin levels were increased with glibenclamide treatment in diabetic mice, whereas such effect was not observed with PGE. These results indicated that P. grandiflorum enhances the hypoglycemic effects of exogenous insulin without stimulating insulin secretion, indicating that insulin sensitivity is increased in diabetic mice.