条斑紫菜多糖的分离纯化及Mark-Houwink方程参数测定

Purification and Determination of Mark-Houwink Equation Constants of Porphyra yezoensis Polysaccharides

目的:获得均一性的条斑紫菜多糖,测定相应的Mark-Houwink方程参数。方法:条斑紫菜经超临界CO2脱脂、水提、醇沉、脱蛋白和DEAE-52柱层析分级分离,获得条斑紫菜多糖,凝胶渗透色谱法鉴定多糖的均一性并同时测定分子量,利用均一性的条斑紫菜多糖测定其Mark-Houwink方程参数,非均一性的条斑紫菜多糖进行验证。结果:分离到分子量分别为2.2×105、3.8×105和6.1×105的均一性条斑紫菜多糖的组分,确定了条斑紫菜多糖在水溶液中25℃下的Mark-Houwink方程的参数为:K=7.86×10-3,α=0.626。结论:经检验,测定的K和α的值可靠,可用于条斑紫菜多糖分子量的研究。

Objective： To obtain homogeneous Porphyra yezoensis polysaccharides （PYPS） and determine corresponding Mark- Houwink constants. Method： PYPS were obtained as follows： ground and degreased by super critical fluid CO2, extracted in distilled water, and then precipitated by ethanol and purified by way of removing protein with chromatography on DEAE-52 column, Molecular weight and purity were identified by GPC. The Mark-Houwink equation constants were determined by 3 homogeneous PYPS, and validated. Results： Three purified PYPS are obtained with the molecular weight as 2.2 ×10^5, 3.8 ×10^5 and 6.1 ×10^5, respectively. The Mark-Houwink equation constants of PYPS in distilled water at 25℃ are： α=0.626 and K=7.86×10^-3. Conclusion： The obtained K and α value are reliable, was confirmed by PYPS2 assays. The values could be applied in molecular weight studies on PYPS molecular weights.