枇杷核黄酮类物质微波法提取及对羟自由基清除作用研究

Study on Flavonoids Extracted from Loquat Pit by MAE and Hydroxyl Radical Scavenging Effects

本文对微波法提取枇杷核中黄酮类物质进行了研究,通过单因素及L9(34)正交试验,得到最佳工艺条件:m(乙醇溶液)/m(枇杷核)=15,提取功率为296W,时间120s提取3次,50%的乙醇溶液。按此最佳方法总黄酮提取率为1.41%。微波法与传统溶剂提取法比较,具有提取时间短、提取率高等优点。同时研究了该提取物对羟自由基清除作用,黄酮类物质对羟自由基的清除作用呈线性相关,相关系数R2为0.9865。

The paper studied the extracting of flavonoids in loquat pit with microwave assisted extraction （MAE）. The single factor and orthogonal design methods L9（3^4） were applied for the optimal technological conditions： m（ethanol）/m（loquat pit）=15, microwave power 296W and 3 times extracting with microwave for 120 seconds, and 50% ethanol. In these conditions, the yield of flavonoids is 1.41%. As compared with tradition ethanol extraction, the microwave extraction is a rapid method with higher yield. At the same time, a linear relationship exists between scavenging of hydroxyl radical and the extracting of flavonoids in loquat pit （R^2=0.9865）.