杏仁中苦杏仁甙的水提取工艺及其含量的测定

Water Extracting Process for Amygdalin in Almond and Its Content Determination

本文用水为溶剂提取杏仁中的苦杏仁甙,采用紫外分光光度法,以甲醇为溶剂,在检测波长为219nm处测定苦杏仁甙的含量,并讨论了料液比、浸泡时间、提取时间以及提取次数对苦杏仁甙的水提取工艺的影响。结果表明:紫外分光光度法测定杏仁中苦杏仁甙在0.01～0.10mg/ml浓度范围内,线性关系良好(r=0.9996),平均回收率在99.751%～102.124%,相对标准偏差为0.9749%,可见此测定法快速、简便、稳定、准确、可靠;水提取杏仁中苦杏仁甙时,影响提取效果的主次顺序为提取时间>料液比>浸泡时间。最佳提取参数为:提取时间20min,料液比1:10,浸泡时间3min,提取2次。

In this paper, amygdalin was extracted by water and determined by ultraviolet spectrophotometry. The solvent was methanol, the detection wavelength was 219nm. And the effects of the ratio between amygdalin and water as extracting solvent at different solid-liquid rates, the soaking time and the length of extracting time were discussed. The experimental results indicated that the concentration is in the range of 0.01 -0.10mg/mg with the r as 0.9996. The recovery is from 99.751% to 102.124%, and the coefficient of variation is 0.9749%. This method is rapid, simple, stable and accurate, reliable; the effectiveness orders with different ratios of amygdalin and water are： extracting time 〉 solid-liquid ratio 〉 soaking time. The optimum technological conditions are： extracting time 20min, solid-liquid ratio 1：10, soaking time 30min and 2 times extract.