摘要
利用反相离子对色谱法(RP-IPC)对啤酒中的腺嘌呤(Ade)、鸟嘌呤(Gua)、黄嘌呤(Xan)和次黄嘌呤(Hyp)等4种嘌呤类物质的测定方法进行了研究。结果表明,当检测波长254nm,流动相为水:甲醇:冰乙酸:四丁基氢氧化铵为883.5:100:15:1.5(V/V/V/V),流速为1ml/min时,方法精密度为1.41%~2.42%,测得四种嘌呤物质的回收率在91.2%~100.3%之间。啤酒中的含嘌呤类物质需要经高氯酸水解成嘌呤,经优化的水解条件为啤酒:高氯酸为1:2、100℃水解30min。
Four purine bases were separated by reversed-phase ion pair chromatography. The optimized conditions were: 254 nm by UV detector with water: methyl alcohol :acetic actd: tetrabutylammonium hydroxide (883.5:100:15:1.5 respectively) as mobile phase and flow rate at lml/min. The average recoveries of four purine bases was 91.2%- 100.3%, with the accuracy of the method as RSD of all four purine bases around 1.41%- 2.42%. Purine compounds in beer were pre-hydrolyzed to purines by perchlorate acid, with the optimal conditions as beer: perchlorate acid (1:2) for 30min at 100℃.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2006年第9期219-222,共4页
Food Science
关键词
啤酒
反相离了对色谱
嘌呤类物质
水解
beer
reversed-phase ion pair chromatography
purine
hydrolysis