反相离子对色谱法测定啤酒中的嘌呤类物质

Determination of Purine Compounds in Beer by Reversed-phase Ion Pair Chromatography

利用反相离子对色谱法(RP-IPC)对啤酒中的腺嘌呤(Ade)、鸟嘌呤(Gua)、黄嘌呤(Xan)和次黄嘌呤(Hyp)等4种嘌呤类物质的测定方法进行了研究。结果表明,当检测波长254nm,流动相为水:甲醇:冰乙酸:四丁基氢氧化铵为883.5:100:15:1.5(V/V/V/V),流速为1ml/min时,方法精密度为1.41%～2.42%,测得四种嘌呤物质的回收率在91.2%～100.3%之间。啤酒中的含嘌呤类物质需要经高氯酸水解成嘌呤,经优化的水解条件为啤酒:高氯酸为1:2、100℃水解30min。

Four purine bases were separated by reversed-phase ion pair chromatography. The optimized conditions were： 254 nm by UV detector with water： methyl alcohol ：acetic actd： tetrabutylammonium hydroxide （883.5：100：15：1.5 respectively） as mobile phase and flow rate at lml/min. The average recoveries of four purine bases was 91.2%- 100.3%, with the accuracy of the method as RSD of all four purine bases around 1.41%- 2.42%. Purine compounds in beer were pre-hydrolyzed to purines by perchlorate acid, with the optimal conditions as beer： perchlorate acid （1：2） for 30min at 100℃.