咸鱼制品中有机磷农药残留的毛细管GC-NPD法测定

Residue Determination of Organophosphorous Pesticides in Salty Fish Products by Gas Chromatography

由于咸鱼制品加工过程中经常有用敌百虫、敌敌畏和甲胺磷等有机磷溶液浸泡来进行防腐,这类产品严重危害人们生命安全。本研究采用毛细管气相色谱法氮磷检测器(GC-NPD)测定咸鱼制品中敌百虫、敌敌畏和甲胺磷。将鱼肉匀浆后。丙酮/二氯甲烷(1:1)提取,以外标法定量分析。当取鱼组织0.5g时,方法检测限为0.002mg/kg,相对标准差为3.3%～7.0%,回收率75.6%～89.3%,变异系数为1.05%～10.12%。该方法简单、灵敏、准确。

Salty fish products are often antisepticised through being marinated in organophosphorous pesticides such as trichlorphon, dichlovos and methamidophos, which endangers lives of people. A method was introduced in this paper to detect the residues of the three pesticides in salty fish products by GC. Fish tissue was equalized by agitator and was marinated in the solution of acetone/dichloromethane （1：1） that extracted the residue of organophosphorous pesticides in Cirrhinus molitorella fish products-one kind of salty fish products; then the result was analyzed quantificationally by external standard method. The results showed that the minimum detection limit was 0.002mg/kg with high separated degree and wide linear relation if the sample was about 0.5g. The results indicated that the recoveries were from 75.6% to 89.3%, relative standard deviation from 3.3%-7.0% and coefficients of variation range in 1.05%- 10.12%. The method was a quick and practical analysis detection method suitable for the requirement of residue determination of pesticides completely.