银杏中银杏酸诱导人白血病细胞U937凋亡的研究

Apoptosis Study on Human or Leukaemia Cell Line U937 Induced by Ginkgolic Acids from Ginkgo biloba L.

目的:研究银杏酸体外对U937细胞生长的抑制作用,探讨其作用机理。方法:采用MTT法检测银杏酸对U937细胞增殖的影响,激光共聚焦显微镜观察细胞形态的变化,DNA琼脂糖电泳检测其生化特征的改变,流式细胞仪分析细胞凋亡率。结果:U937细胞经银杏酸作用24～48h,细胞的生长明显被抑制;银杏酸作用28h出现了细胞皱缩、核浓缩、体积缩小等明显的凋亡的形态学特征;细胞DNA经琼脂糖电泳可见典型的梯形条带;10.0μg/ml的银杏酸作用40h后,细胞凋亡率为14%。结论:银杏酸对U937细胞具有明显的抗肿瘤活性,其作用机理与诱导细胞凋亡有关。

To investigate the inhibition effects of ginkgolic acids on U937 cells in vitro and its anti-tumor mechanism. Method： The growth inhibition effects of ginkgolic acids on U937 cell were assayed by MTT method, Changes of U937 cell morphologic character were observed under laser focusing microscope. Bio-chemistry characteristics of cell apoptosis was observed by DNA agarose electrophoresis. Apoptosis rate of U937 was analyzed by FCM. Result： Ginkgolic acids shows obvious inhibiting effect on cell growth after culturing U937 cell with it for 24-48h. Exposure U937 cell to ginkgolic acids for 28h, that ginkgolic acids make the U937 nucleolus shrink in volume, darken in color, distort in shape. U937 cells show morphologic characteristics of cell apoptosis. U937 cell DNA appeare DNA ladder type in agarose electrophoresis. Exposing U937 cell to 10.0μg/ml ginkgolic acids for 40h, the apoptosis rate is 14%. Conclusion： Ginkgolic acids show an anti-tumor activity through inducing apoptosis in tumour cell.