多发性硬化患者中IL-12和孕酮的检测及其临床意义

The assay and its clinical significance about progesterone and interleukin-12 in the patients with multiple sclerosis

目的检测IL-12和孕酮在多发性硬化(MS)患者中的水平,探讨男、女性多发性硬化患者存在的发病机制,比较男、女性MS患者在临床上发病的差异。方法采用酶联免疫(ELISA)法检测60例MS患者的脑脊液和40例患者血液中IL-12的水平,应用放射免疫法测定40例MS患者血液中孕酮的含量,并与40例正常对照组比较(以上标本男女各半)。结果男、女性MS患者血液中和脑脊液IL-12的含量明显高于正常对照组(P<0.01);女性患者血液中孕酮的含量明显低于正常对照组(P<0.01),男性患者血液中孕酮的含量与正常对照组差别不明显(P>0.05);女性患者血液中孕酮对IL-12的含量存在负相相关作用(r=-0.80,P<0.01),男性相关作用不明显(r=-0.38,P>0.05)。结论IL-12参与多发性硬化的发病过程;孕酮可能是男、女性患者发病差异性的原因之一。

Objective To detect the contents of IL-12 and progesterone and probe into the pathogenesis in the patients and compare the difference between male and female patients with multiple sclerosis. Methods IL-12 in the CSF with 60 patients and blood serum with 40 patients was assessed by enzyme-linked immunosorbent assay （ELISA） and progesterone in the blood serum with 40 patients by radio-immunity（RI）. We compared them with the 40 control subjects and half of them were male patients. Results The levels of IL-12 in CSF and blood serum were significantly higher in the patients than those in the controls（P〈0. 01 ） The progesterone of blood serum in the female patients was significantly lower than that in the control（P〈0. 01 ）. There was no significant difference in the progesterone level between the male patients and the controls（P〈0. 05 ）. There was negative correlation between progesterone and IL-12 in the blood serum of female patients （r = - 0. 80,P〈0. 01 ）. The correlation about male patients is not obvious（r= - 0. 38,P〈0. 05） o Conclusions IL-12 may have an important rate in the patbogenesis of MS and progesterone may be one of the causes of difference between males and female patients.