Ezrin在散发性结直肠癌中的表达及意义

Expression and significance of ezrin in human primary sporadic colorectal carcinoma

背景与目的:细胞骨架和细胞浆膜的连接蛋白Ezrin(埃兹蛋白)被认为可能参与肿瘤的发生发展,是近年来肿瘤转移的研究热点,但Ezrin与结直肠癌发生发展的关系尚不明确。本研究探讨Ezrin在散发性结直肠癌中的表达情况及其临床病理意义。方法:应用EnV ision免疫组织化学的方法检测132例散发性结直肠癌及43例癌旁正常肠粘膜中Ezrin的表达情况,并对其有无转移(转移组74例,未转移组58例)进行分组比较。结果:①Ezrin在结直肠癌中的阳性率(79.5%)显著高于癌旁正常肠粘膜的阳性率(11.6%)(P<0.001);②Ezrin总阳性率在转移组(86.5%)高于未转移组(70.7%)(P=0.026),其中在膜的表达率转移组(31.1%)显著高于未转移组(6.9%)(P<0.001);③Ezrin在结直肠癌中的表达与年龄、性别、肿瘤大小、发生部位、分化程度、浸润深度等临床病理参数均无关(P>0.05)。结论:Ezrin的表达增高与结直肠癌转移密切相关,其从胞质表达到胞膜表达在肿瘤细胞转移过程中可能起着至关重要的作用,并可作为临床判断散发性结直肠癌转移及预后等生物学行为的重要参考指标。

Background and purpose： The membrane cytoskeletal crosslinker--Ezrin may be involved in several functions including cell adhesion, motility and cell survival. There is increasing evidence that it regulates tumor progression. However, the role of Ezrin in the carcinogenesis, progression and metastasis of primary sporadic colorectal carcinoma is still under investigation. This research is to study the expression, the localization and the clinical significance of Ezrin in human sporadic colorectal carcinoma （SCRC）. Methods： Immunohistological EnVision staining was used to detect the expression of Ezrin in 132 cases of human sporadic colorectal carcinoma and 43 adjacent normal colorectal mucosa, and the different expressions of Ezrin among metastatic and non-metastatic SCRC, including 74 metastatic cases and 58 non-metastatic cases were also under investigation. Results： ① The expression rate of Ezrin in SCRC was significantly higher than that in adjacent normal colorectal mucosa （79.5 % Vs 11.6%, P 〈 0.001） ;② The total expression rate of Ezrin was 86. 5% and 70.7%, respectively, （P =0. 026）; the membrane expression rate of Ezrin was 31.1% and 6.9% respectively （P 〈0. 001） in metastatic group and non-metastatic group; ③ There was no relationship between the expression of Ezrin and age, sex, tumor size, location, differentiation of malignancy and invasion depth. Conclusions： The increase in expression of Ezrin was closely correlated to metastasis in human sporadic colorectal carcinoma. The translocation of Ezrin from the cytoplasm to the membrane is correlated with the metastasis. It might be served as an important factor for determining tumor biological behavior.