摘要
背景与目的:骨肉瘤转移机制密切相关的基因筛选是骨科领域研究的难点。本研究应用基因芯片技术筛选高低不同转移特性骨肉瘤细胞亚系的差异表达基因,并探讨其转移的分子机制。方法:提取骨肉瘤细胞亚系A1和A2的总RNA,反转录制备杂交探针,运用基因芯片进行杂交,杂交信号用Agilent Scanner扫描,用Im aGene 3.0软件和Genespring软件分析和处理数据。结果:对A1和A2的基因表达谱分析,发现两者表达差异显著的基因有222个,其中在A1中119个基因表达上调,103个基因表达下调,这些基因可以划分为6个主要功能群,其中49个基因的差异表达非常显著。结论:A1和A2存在多方面基因的表达差异,只有部分差异与骨肉瘤的转移机制密切相关;基因芯片技术能有效地分析各细胞亚系的基因表达谱、为研究骨肉瘤转移机制提供新的途径。
Background and purpose： The screening of the genes being related closely with the mechanisnt of osteosarcoma metastasis was a difficult point in the realm of orthopaedics. We sereened differential expression gene of hmnan osteosarcoma MG-63 cell sublines with different metastatic capabilities with cDNA microarray, and studied the molecular mechanism of osteosarcoma metastasis. Methods： Total RNA of human osteosarcoma MG-63 cell sublines A1 and A2 was extracted , purified to mRNA and then reversely transcripted to cDNA probe respectively. The cDNA probe of A1 was labelled with Cy3 and the cDNA probe of A2 was labelled with Cy5. The two samples were hvbridized with the cDNA microarray. The hybridization signals were scanned by Agilent Scanner and obtained data were analyzed using lma Gene 3.0 software and Genespring software. Results： 222 differential expression genes were found between cell suhlines A1 and A2 by analyzing gene expression profile. There were 119 upregulated genes and 103 downregulated genes in cell subliMes A1. All differential expression genes belonged to six main function groups and 49 genes of these had very obvious differentce in expression. Conclusions： There were many differently expressed genes between A 1 and A2 cell sublimes and only part of them uere closely assoeiated with mechanism of osteosarcolna metastasis. The technology of cDNA lnieroarray could analyze effectively, gene expression profile of human osteosarcoma MG-63 cell snblines, and supply a new approach to study the mechanism of osteosarcoma metastasis
出处
《中国癌症杂志》
CAS
CSCD
2006年第10期841-847,共7页
China Oncology
基金
国家重点基础研究发展规划973项目(No:2002CB513107)
