同种异体移植骨髓基质干细胞修复大鼠急性化学性肝损伤的组织学观察

Histological observations of chemically induced acute hepatic injury repaired by allogeneic bone marrow stem cell transplantation

目的探讨同种异体移植骨髓基质干细胞修复大鼠急性化学性肝损伤的可行性。方法采用CCl4喂养制作SD大鼠急性化学性肝损伤模型,通过肝实质局部注射同种BMSCs(肝损伤移植组),或不行细胞移植(肝损伤对照组)。分别在造模前、BMSCs移植术后6h、1周、5周取肝组织石蜡包埋、切片、HE染色,光镜下观察。结果肝损伤大鼠造模后肝内出现弥漫性分布的点片块坏死,肝血窦充血明显,弥散性炎性细胞浸润。BMSCs移植组大鼠移植术后6h肝内坏死、充血、炎性细胞等改变与肝损伤对照组组织学改变基本类似,移植术后1周、5周上述组织学改变逐渐好转,但BMSCs移植组较肝损伤对照组好转更明显。结论同种异体移植BMSCs有助于修复大鼠急性化学性损伤的肝组织。

Objective To explore the possibility of repairing chemically induced acute hepatic injuries with allogeneic bone marrow stem cell （BMSC） transplantation. Methods A SD rat model of CCl4-induced acute hepatic injury was established, which received transplantation of BMSCs （2.0 ml, 1×10^6/ml） or normal saline injection into the local liver parenchyma, respectively. The rats were sacrificed at 6 h before and 6 h, 1, and 5 weeks after transplantation, and the livers were prepared for microscopic examination. Results Cellular necrosis, bridging necrosis, congestion in the hepatic sinusoid, and inflammatory cell infiltration were seen in the chemically injured livers 6 h after model establishment, and these changes were ameliorated in rats receiving BMSC transplantation. Conclusions Allogeneic BMSC transplantation can repair chemically induced acute liver injuries.