表皮生长因子对小鼠前列腺细胞雌雄激素受体表达的影响

Effects of epidermal growth factor on cellular expression of estrogen and androgen receptors in mouse prostate

目的分析外源性表皮生长因子(EGF)对小鼠前列腺细胞雌雄激素受体表达的影响,探讨EGF在前列腺增生发生中的可能作用。方法实验采用雄性昆明小鼠60只,随机分为1μg/dEGF和2μg/dEGF处理组和对照组,每组20只。实验组和对照组动物分别给予不同剂量的EGF和蒸馏水皮下注射,每日1次,连续28d。采用FCM方法定量分析不同剂量EGF处理组和对照小鼠前列腺细胞雌雄激素受体阳性标记率和表达水平。结果经EGF处理后,前列腺细胞雌激素受体阳性标记率和表达强度均明显升高(P<0.01),2μg/dEGF组雌激素受体表达强度明显高于1μg/dEGF处理组(P<0.01);EGF处理后前列腺细胞雄激素受体阳性标记率和表达强度均明显高于对照组(P<0.05),但不同剂量EGF处理对前列腺细胞雄激素受体表达的影响未见明显差异。结论EGF可调控前列腺细胞雌雄激素受体高表达,可能在前列腺增生的发生中发挥一定作用。

Objective To investigate the effects of epidermal growth factor （EGF）on estrogen receptor （ER） and androgen receptor （AR） in mouse prostate cells and explore the putative role of EGF in prostatic hyperplasia. Methods Sixty male Kunming mice were randomly divided into two EGF groups and one control group （n=20） and subjected to subcutaneous injection of 1 and 2μg/day EGF and distilled water, respectively, for 28 consecutive days. The cellular expression of ER and AR in the prostate of mice in different groups was evaluated by flow cytometry. Results Compared with the control group, the positivity rate of ER and its expression level were significantly increased in the mouse prostate after EGF treatment （P〈0.01）, and the ER expression level was significantly higher in mouse with 2 μg/day EGF treatment than in those treated with 2 μg/day EGF （P〈0.01）. AR positivity rate and expression level also increased significantly in comparison with the control group （P〈0.05）, but no significant variation was found between 1 μg/day and 2 μg/day EGF groups. Conclusion EGF can increase the cellular expression of ER and AR in mice prostate and may play a role in the pathogenesis of prostatic hyperplasia.