CYR61和血管内皮生长因子在结外鼻型NK/T细胞淋巴瘤中的表达及其意义

Expression of CYR61 and VEGF in extranodal nasal-type NK/T cell lymphoma and their significances

目的研究血管诱导生成因子61(CYR61)和血管内皮生长因子(VEGF)在结外鼻型NK/T 细胞淋巴瘤中的表达及其意义。方法应用实时荧光定量 PCR 和免疫组化技术分别检测结外鼻型 NK/T 细胞淋巴瘤中 CYR61 mRNA、VEGF mRNA 及其蛋白水平表达。结果①经实时荧光定量PCR 检测,20例中有19例(95.0%)CYR61 mRNA 表达上调,15例(75.0%)VEGF mRNA 表达上调。②经免疫组织化学染色,40例中有38例(95.0%)肿瘤细胞 CYR61蛋白阳性,25例(62.5%)VEGF 蛋白阳性,与对照组比较差异均无统汁学意义;CYR61和 VEGF 在 mRNA 和蛋白水平表达的一致率分别为95.0%和65.0%;8例患者 CYR61 mRNA、VEGF mRNA 及其蛋白表达一致。③通过生存分析显示CYR61 mRNA 和 VEGF mRNA 表达上调者预后较差,CYR61和 VEGF 表达水平与结外鼻型 NK/T 细胞淋巴瘤的预后相关。结论 CYR61和 VEGF 在结外鼻型 NK/T 细胞淋巴瘤中的表达水平有变化,并与该肿瘤的预后可能有一定的关系。

Objective To investigate the expression of CYR61 and VEGF in extranodal nasal-type NK/T cell lymphoma and its significance. Methods CYR61 mRNA and VEGF mRNA were detected by realtime fluorescence quantitative PCR method in 20 cases of extranodal nasal-type NK/T cell lymphoma. Expressions of CYR61 and VEGF were studied by immunohistochemistry in 40 cases of the tumor. Results ①Overexpression of CYR61 mRNA and VEGF mRNA was found in 19/20（95.0% ） and 15/20（75.0% ） cases, respectively. ②Tumor cells expressing CYR61 protein and VEGF protein were detected in 38 （95.0%） and 25 （62.5%） of the 40 cases respectively, being no significant difference from the control. Co-expression of CYR61 and VEGF at both the mRNA and protein levels was 95.0% and 65.0% , respectively. Over-expression of CYR61 and VEGF at both mRNA and protein levels was found in 8 of the 40 cases. ③ The prognosis of the patients over-expressing CYR61 and VEGF was worse. Conclusion In extranodal nasal-type NK/T cell lymphoma, the expression level of CYR61 and VEGF was changed and it may be of prognostic implication of the tumor.