^(131)I-GM-CSF在人白血病SCID小鼠模型体内的生物学分布

Biodistribution study of ^(131)Ⅰ-GM-CSF in SCID mice bearing human leukemia

目的观察^(131)I-GM-CSF 在人急性髓系白血病 SCID 小鼠模型体内的生物学分布。方法用 SCID 小鼠建立人白血病异种移植模型,用氯胺-T 法制备^(131)I-GM-CSF,观察^(131)I-GM-CSF 在白血病小鼠体内的生物学分布。结果①静脉接种的 HL-60细胞在 SCID 小鼠体内植活,4周后发生白血病;②^(131)I-GM-CSF 主要滞留于模型小鼠的脾脏、骨髓及瘤体组织中,且前两者对^(131)I-GM-CSF 摄取于注入后30min 达峰值,组织摄取率分别为(442.9±86.4)%ID/g 和(4283.8±252.8)%ID/g,滞留24h 后高于其他脏器。而^(131)I 呈非特异性分布。结论 ^(131)I-GM-CSF 集中分布于人白血病 SCID 小鼠模型脾脏、骨髓及白血病细胞浸润组织,具有组织器官相对特异性。

Objective To investigate the biodistribution of ^131I-GM-CSF in SCID mice bearing human AML in vivo. Methods The xenograft model of human leukemia was established in SCID mice. In the leukemia mice,the biodistribution of ^131I-GM-CSF produced by chloamin-T method was studied. Results ①The inoculated HL450 cells could grow in SCID mice,which developed leukemia after 4 weeks. ②131^I-GM-CSF was concentrated in spleen, bone marrow and tumor tissue of the mice. In spleen and bone marrow, ^131I-GM-CSF was uptaken to peak in 30 minutes after injection, the uptaking rate was （442.9 ±86.4）% ID/g and （4283.8 ±252.8）% ID/g, respectively, and maintained on higher level in 24 hours. The injection of ^131I resulted in an even distribution in the whole body. Conclusions 132^I-GM-CSF is able to concentrate electively in spleen, bone marrow and organs infiltrated by leukemia cells. The biodistribution of ^131 I-GM-CSF in the leukemia mice is tissue specific.