转蜘蛛杀虫肽与Bt毒蛋白C肽基因小黑杨对杨扇舟蛾的抗性

Resistance of transgenic Xiaohei poplars with fusion protein gene of the spider insecticidal peptide and Bt-toxin C-peptide to Clostera anachoreta (Fabricius) (Lepidoptera:Notodontidae)

为评价转蜘蛛杀虫肽与Bt毒蛋白C肽融合蛋白基因小黑杨对杨扇舟蛾Clostera anachoreta(Fabricius)的抗性,采用苗木套笼饲喂法和石蜡切片法,对取食转基因小黑杨的杨扇舟蛾幼虫的发育、死亡情况和中肠结构进行了研究。结果表明,杨扇舟蛾幼虫分别取食TT3(转基因无性系3)、TT1(转基因无性系1)和CK(非转基因对照)小黑杨后总历期依次为35.63天、30.39天和28.74天,幼虫化蛹率依次为12.1 %、29.3 %和44.3 %,平均蛹重依次为0.1077g、0.1714 g和0.1893 g。转基因小黑杨能明显延长杨扇舟蛾幼虫的发育历期,降低化蛹率和蛹重。同时,转基因小黑杨有抑制幼虫蜕皮、增加其死亡率和致蛹畸形的作用,且能破坏幼虫中肠,使中肠细胞排列松散、肠腔食物减少、中肠变形,其破坏作用随时间延长而加剧。一般来讲,TT3对杨扇舟蛾的各种影响作用均大于TT1。

For evaluating the resistance of transgenic Xiaohei poplar with fusion protein gene of the spider insecticidal peptide and Bt-toxin C-peptide to Clostera anachoreta （Fabricius）, the development and death of C. anachoreta larvae feeding on different transgenic Xiahei poplars （the 1st transgenic clone, TT1; the 3rd transgenic clone, TT3） or their non-transgenic control （CK） and the histological change in their midgets were investigated in the laboratory through the cage feeding method and oparaffin method. The results indicated that for the larvae fed respectively on TT3, TT1 and CK, the total duration of 5 instars were 35.63 d, 30.39 d and 28.74 d, respectively ; the percentages of pupation were 12.1%, 29.3 % and 44.3 %, respectively ; and the mean weights of pupae were 0.1077 g, 0.1714 g and 0.1893 g, respectively. Feeding on the transgenic plantlets prolonged the larval developmental duration and decreased the pupation rate and pupal weight. At the same time, feeding on transgenic plantlets restrained the ecdyses of larvae, increased their mortality, and resulted in deformation of pupae. Histological examination showed that the midguts of larvae feeding on transgenic plantlets were somewhat damaged, for example, loosing cellular arrangement and decreasing food in midguts and distorting structure, speaking, various effects of TT3 and the damage was more serious with the extension of time. Generally on C. anachoreta were stronger than those of TT1