骨形态发生蛋白-2在诱导兔颅骨再生过程中对转化因子-β1表达的影响

The expression of endogenous TGF-β1 of rabbit pericranium during osteogenesis induced by rhBMP-2

目的研究外源性重组人骨形态发生蛋白-2(rhBMP-2)在诱导兔颅骨再生过程中对内源性转化生长因子-β1(TGF-β1)表达的影响。方法建立颅骨缺损模型,利用免疫组化方法检测缺损区颅骨膜内源性TGF—β1在rhBMP-2作用下的表达情况。结果术后早期实验组颅骨膜聚集的大量间充质细胞即开始表达TGF—β1,且随着间充质细胞的分化表达水平迅速提高,表达高峰出现在术后17,19d,术后7,15,21d实验组和对照组诱导区颅骨膜TGF—β1阳性表达差异有统计学意义(P＜0．01)。结论在诱导颅骨再生过程中,外源性rhBMP-2能诱导并增强内源性TGF-β1的表达。

Objective To study the effect of exogenous rhBMP-2 （recombinant human bone morphogenetic protein-2） on the expression of endogenous TGF-β1 during osteogenesis in rabbits. Methods After successfully duplicating experimental rabbit skull defect model, we detected and analyzed the expression of endogenous TGF-β1 of the pericranium in different stages by using imnmnohistochemical method in both the experimenral group and the control group. Results In the experimenral group a lot of mesenchymal cells with positive expression of endogenous TGF-β1 of the pericranium aggregated in duramater in the early stage after operation, and the expression level increased rapidly with the differentiation of mesenchymal cells, and reached the peak at 17, 19 day postoperation. Positive cell number of TGF-β1 of pericranium was statistically analyzed in both groups at 7, 15, 21 days, showing significant difference （t-test, P 〈0. 01 ）. Conclusion Exogenous rhBMP-2 can induce and enhance the expression of endogenous TGF-β1 during osteogenesis.