大鼠肾小体发育中滤过屏障超微结构的演变

Changes of ultrastructures of filtration membrane during development of rat renal corpuscle

目的:探讨大鼠肾小体发育中滤过屏障超微结构的变化规律。方法:采用光镜、电镜技术,并结合体视学分析方法,对不同发育阶段大鼠滤过屏障超微结构的变化进行形态学观察和体视学测量。结果:在肾小体发育过程中,足细胞和内皮细胞的形状逐渐低平,内皮孔增多,足突及裂孔大量分化增多;足细胞下先出现电子密度较低的基膜,随后内皮细胞下才出现基膜,以后,二者融合形成一层电子密度较高的血管球基膜。结论:在大鼠肾小体的发育过程中,足细胞比内皮细胞先分泌基膜,然后二者融合形成血管球基膜。

Objective： To explore the ultrastructural characters of filtration membrane in the development of the rat renal corpuscle. Methods： Light and electron microscope combined with stereology methods were used to ob serve and measure the ultrastructural characters of filtration membrane at different developmental stages of rats. Results： In the development of the renal corpuscle, the height of podocytes and endothelial cells became markedly reduced, and the number of foot processes and endothelial fenestrae steadily increased. It was the podocyte that firstly formed basement membrane, and then the endothelial cells continued. Fusion of them led to the formation of a thick glomerular basement membrane. Conclusion： In the development of the renal corpuscle, it is the podo- cyte that firstly forms basement membrane, and then epithelial and endothelial basement membrane fuses to form the glomerular basement membrane.