MPTP损伤的小鼠PD模型的制作与评价

The Creation and Assessment of MPTP-Lesioned Model of PD in Mice

帕金森病(Parkinson!sdisease,PD)动物模型研究的目的是揭示多巴胺能神经元特异性损伤的机制,进而探索针对这种损伤的神经保护方法或治疗方法.由神经毒素MPTP损伤的小鼠PD模型,广泛应用于散发性PD的研究中.根据注射总剂量、两次注射间隔时间、注射方式的不同,制成了适合于不同研究目的的各种小鼠PD模型.关于MPTP导致的PD模型动物神经损伤的评价方式也是多层面、多指标并存的.对MPTP动物模型的起源和MPTP导致多巴胺能神经元损伤途径进行了较为系统的概述,并对MPTP小鼠PD模型的制作方法与评价指标进行较为详细的归纳.

The aim of studying on animal model of Parkinson＇s Disease （PD） is to discover the mechanism underlying the specific lesion of the dopaminergic neurons, then to seek out protective ways or therapies against the lesion. The mouse model of PD induced by neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrapyridine （MPTP） is applied extensively in the studies of the sporadic PD. Based on differences in the total amount of MPTP administrated, time between injections, and the injection style （intraperitoneally versus subcutaneously）, various models of PD, which could satisfy many different studies, have been created. The assessment of the MPTP lesion is multilevel and contains a series of parameters. The origin of MPTP-lesioned model of PD and the pathways of MPTP causing lesions to dopaminergic neurons were systematically illuminated, then the creation and assessment of MPTP-lesioned PD model was elaborated.