不同化疗药物对宫颈癌裸鼠移植瘤微小染色体维持蛋白7表达影响的研究

Experimental study of the inhibition of minichromosomal maintenance 7 expression of different chemotherapeutic drugs on cervical cancer xenografts

目的：研究铂类和氧化嘧啶类药物对HeLa细胞裸鼠移植瘤微小染色体维持蛋白7表达的影响。方法：将HeLa细胞注射于4～6周龄雌性裸鼠皮下建立移植瘤模型，根据所用药物将裸鼠分组为A：对照组、B1：顺铂组、B2：卡铂组、B3：5-FU组、B4：卡培他滨组（capecitabine，商品名：xeloda）、B5：顺铂＋5-Fu组、B6：顺铂＋卡培他滨组、B7：卡铂＋5-Fu组。RT—PCR法检测微小染色体维持蛋白7（MCM7）的表达，并与大体组织的观察指标如肿瘤体积、质量的变化及抑瘤率进行比较。结果：B1、B2、B5、B6、B7的MCM7表达低于A，差异有统计学意义（P〈0．05），B7的MCM7表达低于B4，差异也具有统计学意义（P〈0．05），但析因分析未显示出他们有正交互效应。结论：对宫颈癌移植瘤抑制作用强的药物其MCM7的表达低，MCM7有可能成为评价宫颈癌化疗药物疗效的生物学指标。

Objective：To study the the inhibition somal maintenance 7 （ MCM7 ） of cervical cancer xenografts of expression level of minichromo- treated with platinum and fluoroprimidine drugs. Methods：The HeLa cells were subcutaneous injected into the female nude mice of 4 - 6 weeks of age. The mice were divided into 8 groups according to the drugs, namely, A ： control group, B1 ： cisplatin, B2 ： carboplatin, B3 ： 5-FU, B4 ： capecitabine, B5 ： cisplatin ＋ 5-FU, B6：cisplatin ＋ capecitabine, B7： carboplatin ＋ 5-FU. MCM7 expression was evaluated by RT- PCR, and was compared to the parameters, such as the volume and weight of the tumors and the inhibition ration. Results： The MCM7 expression of group B 1. B2. B5. B6. B7 was significantly less than that of control group（ P 〈 0.05 ）. On the other hand ,the MCM7 expression of group B7 was also significantly less than that of B4 （ P 〈 0.05 ）. However, there was no interaction effect between B7 and B4 by muhifactofial analysis. Conclusion ：The drug that had stronger inhibition effect might be had lower expression of MCM7, MCM7 may be a new indicator to evaluate the curative effect of chemotherapy on cervical cancer.