罗勒多糖对卵巢癌生物学行为的影响及其机制研究

The effect and mechanism analysis of basil polysaccharide against ovarian cancer

目的:观察中药罗勒多糖对卵巢癌生物学行为的影响,探讨其抗卵巢癌作用的可能机制。方法:建立荷NuTu-19卵巢癌大鼠模型,观察罗勒多糖对荷瘤鼠肿瘤生物学行为的影响;抽提肿瘤组织总RNA,逆转录标记cDNA探针,用Cy3-dUTP标记对照组,Cy5-dUTP标记实验组,与表达谱芯片进行杂交,ImaGene3.0软件分析统计。同时取肿瘤组织,进行常规病理学检测。结果:与对照组相比,罗勒多糖治疗组肿瘤的生长及转移行为都受到明显的抑制:肿瘤数量减少,体积减小,腹水量显著减少(P<0.05);腹腔脏器转移程度积分显著降低(P<0.01)。罗勒多糖作用后,卵巢癌的基因表达谱发生了明显变化,应用表达谱芯片共筛选出168条差异表达基因。结论:罗勒多糖通过多个药效靶点抗肿瘤增殖及转移。

Objective： To investigate the effect of basil polysaccharide on ovarian carcinoma and analyze the potential mechanism using gene expression microarray. Methods：Rat models bearing NuTu-19 cells were established and treated with basil polysaccharide. The changes of tumor cell biological activity were observed. Total RNA of the tumor tissue was extracted and labeled with Cy3-dUTP or with Cy5-dUTP, then hybridized with the microarrays. The chips were scanned with a ScanArray and the acquired images were analyzed using ImaGene 3.0 software. Pathology study was also carried out. Results：Compared with the control, the amounts of ascites of the rat treated with basil polysaccharide were decreased （ P 〈 0.05 ） and the score of abdominal carcinoma metastasis degree was decreased significantly （ P 〈 0.01 ）. One hundred sixty-eight differently expressed genes were found in the tumor specimen dealed with basil polysaccharide. Conclusions： Basil polysaccharide may have multi-targets during the anti-cancer metastasis process.