大鼠体内IGF-Ⅰ水平改变与心肌梗死的关系

Changes of Blood and Myocardial Tissue Contents of IGF-Ⅰ After Development of Acute Myocardial Infarction in Rat Models

目的:为探讨胰岛素样生长因子-Ⅰ(IGF-Ⅰ)在急性心肌梗死大鼠体内的变化及其与急性心肌梗死(AM I)病程的关系。方法:将Sprague-Daw ley(SD)大鼠制成AM I模型,分成M I2d和M I14d组,每组8只。分别于心肌梗死后2d和14d处死大鼠,采用放射免疫分析测定大鼠血液和心肌组织匀浆中的IGF-Ⅰ含量,并与正常对照组比较。结果:心肌梗死后2d时大鼠体内IGF-Ⅰ均高于正常组,但无统计学意义(P>0.05)。14d时血液和心肌组织中的IGF-Ⅰ显著高于正常组和2d组(均为P<0.05),正常组和14d组血液和心肌中的IGF-Ⅰ水平之间有显著相关性(r=0.9992,r=0.9987,P<0.01)。结论:心肌梗死后大鼠血液和心肌组织中IGF-Ⅰ水平较正常组高,并随心肌梗死的病程而逐步上升。

Objective To study the changes of IGF - Ⅰ contents in blood and myocardium after experimental acute myocardial infarction in rat models. Methods Rat models of acute myocardial infarction were prepared with intraperitoneal injection of isoproterenol. Eight models were sacrificed 48h later and another 8 models were sacrificed 14 days after preparation. Serum and myocardium homogenate contents of IGF - Ⅰ were measured with RIA in these models as well as 8 control rats. Results The serum and myocardial contents of IGF - Ⅰ increased in the models sarcrificed at 48h, but were not signiificandy higher than those in the controls （P 〉 0.05）. At 14^th day, the levels were significantly higher than those in controls and at 48h （both P 〈 0.05）. The serum and myocardial contents of IGF - Ⅰ were mutually correlated in the controls and 14 day models （r = 0.9987, r = 0.9992; P 〈 0.01）. Conclusion After myocardial infarction, the serum and myocardial IGF - Ⅰ contents increased along with the course of disease in the rat models.