鼻咽癌患者血清TGF-β_1、IL-8水平和T淋巴细胞亚群水平测定的临床意义

Clinical Significance of Determination of Changes of Serum Contents of TGF-β_1,IL-8 and T Cell Subsets Distribution Type in Patients with Nasopharangeal Carcinoma

目的:探讨了鼻咽癌患者血清TGF-β1、IL-8和T淋巴细胞亚群水平的变化及意义。方法:分别应用放免法、酶联免疫法和单克隆抗体测定对31例鼻咽癌患者进行了血清TGF-β1、IL-8水平和T淋巴细胞亚群的测定,并与35例正常健康人作比较。结果:鼻咽癌患者血清TGF-β1、IL-8和CD8水平非常显著高于正常人组(P<0.01),而CD3、CD4、CD4/CD8比值又非常显著低于正常人组(P<0.01)。鼻咽癌患者血清TGF-β1水平和CD4细胞数量及与CD4/CD8比值呈负相关,与CD8细胞呈正相关。结论:观察鼻咽癌患者血清TGF-β1、IL-8水平和T淋巴细胞亚群水平的改变有助于鼻咽癌的诊断及对预后的评价。

Objective To explore the significance of changes of serum transform growth factor β1 （TGF - β1） and IL - 8 as well as T cell subsets distribution type in patients with nasopharangeal carcinoma. Methods Serum TGF - β1 （with ELISA）, IL - 8 （with RIA） levels and T cell subsets distribution type （with monoclonal antibody technique） were determined in 31 patients with nasopharangeal carcinoma as well as in 35 controls. Results The serum levels of TGF - β1, IL - 8 and CD8 percentage were significantly higher in the patients than those in controls （P 〈 0.01）, while CD3, CD4 percentage and CD4/CD8 ratio were significantly lower （P 〈 0.01）. In the patients, the serum TGF - β1 levels were positively correlated with CD8 percentage and negatively correlated with CD4 percentage and CD4/CD8 ratio. Conclusion The altered levels of TGF - β1 and IL - 8 as well as the decrease of CD4/CD8 were correlated with the clinical development and prognosis in patients with nasopharangeal carcinoma.