高效液相色谱法测定直肠癌术中区域化疗血液、盆腔液和组织5-氟尿嘧啶浓度

Determination of 5-FU in Serum,Peritoneal Fluid and Tissue by HPLC in Patients Undergoing Rectal Cancer Operation and Receiving Regional Chemotherapy

目的:测定直肠癌术中区域化疗血液、盆腔液和组织5-氟尿嘧啶浓度。方法:20例直肠癌患者术中行区域化疗,注药后2、5、10、20、30、60min采集门静脉血、周围静脉血、盆腔液和癌旁组织,以高效液相色谱法测定5-氟尿嘧啶浓度。结果:各样本注药后2min即出现峰浓度,此后逐渐降低。其中,盆腔液浓度最高,癌旁组织最低,门静脉血药浓度高于周围静脉血。结论:直肠癌术中区域化疗,可使盆腔液、门静脉血和癌旁组织维持高的药物浓度,这对预防直肠癌术后局部和肝脏转移有指导意义。

OBJECTIVE： To determine 5- Fu concentration in serum, peritoneal fluid and tissue by HPLC in patients undergoing rectal cancer operation and receiving regional chemotherapy. METHODS： 20 patients who undergoing rectal cancer operation and receiving regional chemotherapy were administered with drugs, whose blood samples were taken from portal veins, peripheral veins, peritoneal fluid and tissues around tumor at 2, 5, 10, 20, 30 and 60rain, respectively after administration for the determination of 5- FU by HPLC. RESULTS： Peak concentrations in all samples appeared 2 minutes after administration, but lowered gradually thereafter. The drug level in peritoneal fluid was the highest, but was the lowest in the tissue, and that in the portal vein was higher than in peripheral veins. CONCLUSION： For patients who undergoing rectal cancer operation, regional chemotherapy could maintain 5- FU concentration at a higher level in portal vein, peritoneal fluid and tissue around cancer, which has significance in the prevention of metastasis to the local sites and liver after rectal cancer operation.