大肠癌及癌周粘膜酸性粘液物质组织化学观察

Histochemical observation on sulphated and non- sulphated mucins in colorectal adenocarcinoma and mucosa around cancer

采用醛复红-阿辛蓝粘液染色法,同时显示硫酸及非硫酸化粘液,对不同组织类型的结肠癌、癌周粘膜及腺瘤的粘液反应进行比较研究。结果表明。管状腺癌及乳头状腺癌癌区粘液表达为阴性或其腺腔内有少量非硫酸化粘液;粘液腺癌有一定的粘液表达;印戒细胞癌癌细胞及粘液池有大量非硫酸化粘液表达。癌周粘膜以非硫酸化粘液为主,近癌区腺体粘液显著减少。癌区及癌周粘膜粘液反应与正常肠粘膜的反应截然不同,但与人类胚胎早期粘蛋白相似。比较腺瘤及腺癌的粘液反应表明,前者反应多种多样。但硫酸化粘液明显较腺癌多,当发生不典型增生时,阴性表达大大增加,粘液以非硫酸化粘液为主,符合由结肠腺瘤在不典型增生的基础上发生恶变的渐进过程。

AF/AB ( Aldehyde Fuchsin-Alcian Blue ) staining technique was employed in the experiment to reveal sulphated and non-sulphated acid mucins simultaneously. Comparative studies of mucinous reaction on colorectal adenocarcinoma of different histologic types, mucosa around cancer, and adenoma of colon were carried out. The results showed that the mucinous reaction in tubular and papillary adenocarcinoma expressed negative or little non-sulphated mucins in the lumen of cancerous glands; mucous adenocarcinoma had more or less mucinous expression; signet-ring cancer cells and mucinous pools were rich in non-sulphated mucins. The mucins in mucosa around cancer were ' predominated by non-sulphated mucins. In the glands directly adjacent to carcinoma, mucins were significantly diminished. The mucinous reactions of cancer and mucosa around cancer differed tremendously from that of normal colonic mucosa, but were very similar to early human embryo colonic mucins. When malignant or atypical hyperplasia occurs,secretion is scanty or absent in most cases, and is expressed mostly as non-sulphated mucins. It is implied that the differentiation of the cells becomes poorer and is corresponding to a gradual transition from adenoma via atypical hyperplasia to cancer.