HPLC测定肠炎消中小檗碱含量

Determination of Berberine in Changyanxiao Solution by HPLC

目的建立肠炎消中小檗碱含量测定方法。方法HPLC，色谱柱为Diamonsil C18；以乙腈-0．01mol·L^-1庚烷磺酸钠与0．02mol·L^-1。磷酸二氢钾等量混合溶液（用10％磷酸调节pH值3．0）（32：68）为流动相，检测波长为265nm。结果小檗碱在0．0744-0．744μg范围内线性良好，相关系数r=0．9999，平均回收率为99．4％（RSD=1．57％）。结论本法快速、准确，重复性好，可用于肠炎消的质量控制。

Objective To establish a HPLC method for the quantitative determination of berberine in changyanqing solution. Methods Determination was performed on the Diamonsil C18 column（4.6 mm ×250 mm,5μm）, with acetonitrile and a mixed solution of equal amount of 0.01 mol · L^ -1 Sodium heptanesulfonate and 0.02 tool · L^-1 potassium dihydrogen phosphate （ pH was adjusted to 3.0 by 10% phosphoric acid） （32： 68） as the mobile phase, and the wavelength was set at 265 nm. Results The linear range of the determination is 0.0744 -0.744 μg（r =0.9999）, the average recovery was 99.4% with RSD 1.57% for berberine. Conclusion The method was specific, accurate and simple . It may be used for the quality control of changyanxiao solution.