热休克蛋白70肿瘤多肽复合物抗胶质瘤免疫效应的研究

Immunal Effect of Anti-glioblastoma by Heat Shock Protein70 Associated Tumor Peptides,Purification from G422

目的探讨从小鼠胶质瘤组织中提取的热休克蛋白70肿瘤肽复合物(HSP70-PC)及其抗胶质瘤免疫保护效应.方法自小鼠胶质母细胞瘤G422肿瘤组织提取HSP70-PC,小鼠分别以HSP70-PC(10μg/只)、细胞裂解液(10μg/只)免疫后分别在其颅内和皮下接种肿瘤,比较颅内荷瘤组与未免疫组的成瘤率及生存时间;皮下荷瘤组与未免疫组的成瘤率、肿瘤重量,并应用流式细胞仪技术测定小鼠心腔血中淋巴细胞百分比、CD3+CD8+和CD3+CD4+T淋巴细胞的百分比.结果颅内荷瘤组小鼠成瘤率为100%、100%、83.3%,平均生存期为(16.00±0.58)d、(18.70±1.33)d、(25.33±1.86)d;皮下荷瘤组小鼠成瘤率为100%、100%、33.3%,肿瘤重量为(6.00±1.67)g,(2.30±1.23)g,(0.12±0.20)g,流式细胞仪检测小鼠淋巴细胞群为(32.65±9.06)%、(51.30±7.72)%、(67.42±6)%;CD3+CD8+T淋巴细胞为(4.30±1.97)%、(7.06±2.20)%、(11.75±3.57)%;CD3+CD4+T淋巴细胞为(14.28±6.75)%、(17.17±5.04)%、(22.5±5.18)%.结论热休克蛋白70肿瘤肽复合物可诱导特异性细胞毒性T淋巴细胞(CTL)反应,延长小鼠生存期,具有明显的抗胶质瘤免疫保护作用.

Objective Purify heat shock protein70 - associated tumor peptides （ HSP70 - PC ） from G422 glioblastoma, mice were immunized with HSP70 - PC, investigate its immunoprotective effect against glioma on mouse in vivo. Methods HSP70 - PC was acquired from G422 glioblastoma. After immunization with HSP70 -PC （10 μg per mouse） and cell lysate （10 μg per mouse） vaccine respectively, mice were inoculated with G422 tumor cells after two weeks. The progression of tumor and life span of mice were tested. Results In mice model of intracranial G422 ： the rate of tumor formation was 100%, 100%, 83.3%, the MST （ mean survival time） was （ 16. 00 ± 0. 58） d, （ 18.70 ± 1.33 ） d, （25.33 ± 1.86） d ; in mice model of G422 ： the rate of tumor formation was 100%, 100% , 33.3%, the weight of tumor was （6. 00 ± 1.67 ） g, （2. 30 ± 1.23 ） g, （0. 12 ± 0. 20） g, and the result of flow cytometric analysis of the blood of mice showed that lymphocytes was （32.65 ±9.06）%, （51.30 ±7.72）%, （67.42 ±6.00）%; CD3^＋ CD8^＋ T lymphocytes was （4.30 ± 1.97） %, （ 7.06 ± 2.20 ） %, （ 11.75 ± 3.57 ） % ; CD3^＋ CD4^＋ T lymphocytes was （ 14. 28 ± 6. 75）%, （17.17 ± 5.04）%, （22.5 ± 5. 18）%. Conclusion HSP70 -PC can induce response of CTI, and prolong the life - span of mice, and have obviously immunoproteetive effect against glioma.