血清HBVDNA与乙型肝炎病毒血清学标志物的关系

Relationship between serum HBVDNA and HBV serum markers

目的探讨荧光定量聚合酶链反应（FQ-PCR）技术定量检测HBV DNA与乙型肝炎病毒（HBV）血清学标志物之间的关系。方法采用ELISA检测乙肝病毒血清学标志物，FQ-PCR法检测HBVDNA，比较不同乙肝病毒血清学标志物阳性组合HBVDNA阳性情况。结果HBsAg、HBeAg和HBcAb性组HBVDNA阳性率为100％（155／155）；HBsAg、HBeAb和HBcAb阳性组HBV DNA阳性率为35．2％（50／142）；HBsAg、HBcAb阳性组HBV DNA阳性率为30．2％（38／126），HBsAg阳性组血清HBVDNA阳性率为10．0％（3／30）。结论荧光定量PCR法检测血清中衄VDNA含量可以准确地反映病人体内病毒的感染和复制情况，可准确地为临床提供科学的诊断依据。

Objective To explore the relationship between serum HBV serum markers and HBVDNA. Methods Serum HBV markers were determined by ELISA, and HBVDNA was determined by FQ-PCR. Results I-IBVDNA was 100% positive （155/155） in HBsAg, HBeAg and HBcAb positive group; 35.2% （50/142） in HBsAg, HBeAb and HBcAb positive group; and 10.0% （3/30） in HBsAg positive group. Conclusion Serum HBVDNA level determined by FQ-PCR could reflect status of HBV infection and replication, and could supply evidence fro clinic diagnosis.