摘要
选用铜绿假单胞菌(Pseudomonas aeruginosa,PAO1)为研究菌株,通过实验发现在该菌种自身生长过程中同时产生一种抑制剂,该种抑制剂对革兰氏阴性菌的AHL型信号分子有明显抑制作用。其优化发酵条件为:3 g/L的葡萄糖为碳源,3 g/L的硫酸铵为氮源,250 mL三角瓶中装液量为150 mL,37℃摇床发酵培养72 h。
Pseudomonas aeruginosa (PAO1) is selected as the experimental bacterium. It reveals that a quorum sensing inhibitor appears in the bacterium growing process, which markably inhibites the activity of AHI.s in the gram-negative bacteria. The optimum fermentation condition for this inhibitor by PAO1 is as follows: glucose is used as carbon source and (NH4)2SO4, as nitrogen source; both concentration, 3 g/L; loading volume in 250 mL flasks, 150 mL; incubated temperature, 37℃; cullure lime, 72 h.
出处
《大连轻工业学院学报》
2006年第3期183-186,共4页
Journal of Dalian Institute of Light Industry
关键词
信号分子抑制剂
提取
发酵
quorum sensing inhibitor
extraelion
fermentation