分子信号抑制剂的提取及其优化发酵条件

Extraction of quorum sensing inhibitor and its optimal fermentation condition

选用铜绿假单胞菌(Pseudomonas aeruginosa,PAO1)为研究菌株,通过实验发现在该菌种自身生长过程中同时产生一种抑制剂,该种抑制剂对革兰氏阴性菌的AHL型信号分子有明显抑制作用。其优化发酵条件为:3 g/L的葡萄糖为碳源,3 g/L的硫酸铵为氮源,250 mL三角瓶中装液量为150 mL,37℃摇床发酵培养72 h。

Pseudomonas aeruginosa （PAO1） is selected as the experimental bacterium. It reveals that a quorum sensing inhibitor appears in the bacterium growing process, which markably inhibites the activity of AHI.s in the gram-negative bacteria. The optimum fermentation condition for this inhibitor by PAO1 is as follows： glucose is used as carbon source and （NH4）2SO4, as nitrogen source; both concentration, 3 g/L; loading volume in 250 mL flasks, 150 mL; incubated temperature, 37℃; cullure lime, 72 h.