人参中8种皂苷的HPLC测定

The Determination of Contents of 8 Ginsenosides in Extraction of Panax ginseng by HPLC

目的：为8种人参皂苷的含量测定建立一套方法。方法：色谱柱：ODS柱（Kromasil 250mm×4．6mm，5μm），DAD检测器；流动相：A为乙腈，B为水，梯度洗脱0～35min 19％A，35—55min 19％～29％A，55～75min 29％A，75—100min 29％～40％A。流速：1ml／min，温度：室温，检测波长：203nm。结果：用本方法对各种皂苷的测定结果：稳定性试验RSD为0．55％～2．26％，精密度试验RSD为0．85％～1．93％，重复性试验RSD为0．97％～2．72％。结论：本法稳定性好，精确度高，重复性好，可作为测定这8种皂苷的一个较好的方法。

Objective： To establish a method to determine the contents of 8 ginsenosides in extraction of Panax ginseng by HPLC. Methods： The sample was analyzed on an ODS chromatogram column （ Kromasil 250mm ×4. 6mm,5μm）, with mobile phase of acetonitrile-water （ gradient elution） at flow rate 1.0ml/min and detection at wavelength of 203nm. Results： RSD of stability, precision and recurrency was 0. 55% -2.26% , 0. 85% - 1.93% and 0. 97% -2.72% respectively. Conclusion： This method can be good for the content determination of ginsenoside.