维肝力对贫血小鼠骨髓有核细胞凋亡及Bcl-2、Bax蛋白表达的影响

Effect of WeiGanLi on Apoptosis and Expression of Bcl-2、Bax Protein in Bone Marrow Nucleated Cells of Anemic Mice

目的:研究复方维肝力对贫血小鼠骨髓有核细胞(BMNCs)凋亡及凋亡相关蛋白Bc l-2、Bax表达的影响,探索维肝力抑制贫血小鼠骨髓有核细胞凋亡的机制,发掘维肝力在临床上的新用途。方法:观察维肝力对贫血模型小鼠外周血象、骨髓有核细胞数的影响;用流式细胞仪检测贫血小鼠BMNCs细胞周期;用TUNEL法检测贫血小鼠BMNCs的凋亡;用免疫组化SABC法检测BNMCs中Bc l-2及Bax蛋白的表达。结果:维肝力能显著提高骨髓抑制贫血小鼠外周血红细胞、白细胞、血红蛋白、血小板以及BMNCs数目;可明显促进BMNCs从静止期进入细胞周期;能明显减少接受2.0Gy60Coγ射线辐射后诱导的BNMCs凋亡。同时,维肝力能使Bc l-2蛋白表达上调,而Bax蛋白表达则下调。结论:维肝力可能是通过上调Bc l-2的表达及降低Bax的表达,从而有效抑制辐射所致小鼠骨髓细胞的凋亡,保护造血细胞使其免受损伤,有利机体造血功能的恢复。

Objective： To study the effect of WeiGanLi on apoptosis and expression of Bel-2 ,Bax protein in bone marrow nucleated cells （BMNCs） of anemic mice and investigate its mechanism, so as to find its new plinicuse. Methods： Effects of WeiGanLi on the amount of the peripheral blood cells and BMNCs were determined on the model of myelosuppressed anemic mice. The cell cycle of BMNCs was analyzed by flow cytometer. The apoptosis of BMNCs was detected by TUNEL, and the expression of Bcl-2 and Bax protein were detected by immunohistochemical method. Results： WeiGanLi could significantly increase the number of RBC, WBC, HGb, Pit as well as the number of BNMCs, significantly active BNMCs from stasis into cell cycling and Markedly decrease tbe apoptosis of BNMCs after 2. OGy ^60Coγ irradition while the expression of Bcl-2 increased and Bax decreased. Conclusion： WeiGanLi can significantly inhibit the apoptosis of BMNCs probably due to its enhancing effects on Bcl-2 expression, and inhibitory effect on Bax expression. In this way, it prevents the blood cells from injury, and contributes to the rehabilitation of the organic blood production.