淫羊藿苷和黄芪苷Ⅰ对骨髓基质细胞增殖及成骨能力的影响

The Effect of Icariin and Astragalosid I on the Proliferation and Differentiation of Bone Marrow Stromal Cells

目的:探讨补肾药有效成分淫羊藿苷和黄芪苷Ⅰ对实验犬骨髓基质细胞(BMSCs)增殖及其成骨能力的影响。方法:抽取犬BMSCs,诱导培养、传至第三代时于培养液中添加淫羊藿苷或黄芪苷Ⅰ使两种药物的终浓度为50 ng/m l。设BMP-2阳性对照和空白对照组,分别于第1至第8天每天测细胞的MTT值,绘制BMSCs生长曲线、观察其增殖情况;于第1、3、6、10、14天检测BMSCs的ALP和OD值,绘制该OD值变化曲线判断ALP的活性。结果:淫羊藿苷和黄芪苷Ⅰ预处理后BMSCs的MTT值从第1天至第7天均高于空白对照组;BMSCs分泌ALP的OD值也均高于空白对照组,二者差异有显著性(P<0.05)。结论:淫羊藿苷和黄芪苷Ⅰ能促进BMSCs增殖、促进BMSCs合成ALP从而增强其成骨能力。

Objective： To observe the effects of icariin and astragalosid Ⅰ on the proliferative and alkaline phosphatase （ALP） activity of dog bone marrow stromal cells （BMSCs）. Methods： The dog＇ s BMSCs were isolated and cultured in vitro. The 3th generation BMSCs were treated with icariin or astragalosid Ⅰ at the concentration of 50ng/ml and compared with BMSCs of BMP-2 group and control group. The growth curves of BMSCs were drawn by 3-（4,5-dimethylthiazole-2-yl）-2, 5-biphenyl tetrazolium bromide （MTT） colorimetric assay every day from the 1st to the 8th day to estimate the proliferative ability of BMSCs. The curves of OD value of ALP excreted by BMSCs on the 1st, 3th, 6th, 10th and the 14th day were recorded to estimate the ALP activity of BMSCs. Results： After the pertreatment with icariin and astragalosid Ⅰ, the BMSCs acquired higher MTT values and higher ALP＇ s OD values as compared with control group and the difference between experiment group and control group was statistically significant （ P 〈 0. 05 ）. Conclusion ： Icariin and Astragealosid Ⅰ can accelerate the proliferation and ALP excretion of BMSCs. At the same time, the osteogenesis ability of these cells is greatly improved.