COPD营养不良与细胞因子的关系及消炎痛干预实验研究

Relationship of Cytokine Level with Malnutrition of Chronic Obstructive Pulmonary Disease and Therapeutic Effects of Indomethacin

【目的】探讨慢性阻塞性肝疾病(COPD)营养不良与细胞因子的关系及消炎痛对COPD营养不良的影响。【方法】按气管内滴内毒素和熏香烟法建立COPD大鼠模型,选择营养不良COPD大鼠予以消炎痛干预,检测干预前后营养指标及血清TNF-α、IL-1β的变化,并与生理盐水对照组比较。【结果】消炎痛干预后血清TNF-α、IL-1β水平明显降低,体重、血浆白蛋白、血浆甘油三酯明显升高,血糖明显下降。【结论】消炎痛通过抑制血清TNF-α、IL-1β的升高可以改善COPD营养不良大鼠的营养状况。

[Objective]To explore relationship of cytokine level with malnutrition of rats with Chronic Obstructive Pulmonary Disease （COPD） and therapeutic effects of indomethacin for malnutrition of COPD. [Methods]The rats COPD models were established by intratracheal instillation of lipopolysaccharide （200μg/ 100μl） twice and exposed to cigarette smoke for four weeks, 30 min per day. Indomethacin intervention group was abdominally injected indomethacin （0.7mg/kg）, the intervention duration was 10 days. Serum albumin, glucose.triglyceride. TNF-α, IL-1β levels and body weight of different groups in different stages were measured and compared. [Results]After intervention with indomethacin, the intervention group showed higher serum albumin, triglyceride and body weight levels, lower serum TNF-α, IL-1β glucose levels than group without intervention and the saline control group. The difference was significant. [Conclusion]Indomethacin may improve the nutritional status of malnutrished COPD rats by inhibiting the growth factors of cytokine TNF-α, IL-1β.