含硒停搏液对大鼠心肌缺血／再灌注损伤的保护作用

EFFECTS OF CARDIOPLEGIC SOLUTION CONTAINING SELENIUM ON RATS' MYOCARDIAL ISCHEMIA AND REPERFUSION INJURY PROTECTION

采用Ｌａｎｇｅｎｄｏｒｆｆ灌注模型，对心肌缺血／再灌注损伤中氧自由基清除酶系统超氧化物歧化酶（ＳＯＤ）、谷胱甘肽过氧化物酶（ＧＰｘ）和乳酸脱氢酶（ＬＤＨ）、磷酸肌酸激酶（ＣＫ）、丙二醛（ＭＤＡ）、超微结构的变化及硒的抗氧化效应进行了观察。随缺血时间的延长，ＬＤＨ、ＣＫ活性及ＭＤＡ的含量增加，而ＳＯＤ、ＧＰＸ的活性降低，此时超微结构损伤严重。预先给Ｓｔ·Ｔｈｏｍａ’ｓ停搏液中加入亚硒醚钠再进行缺血／再灌注，冠脉流出液中的ＬＤＨ、ＣＫ活性降低，心肌ＭＤＡ含量减少；而心肌ＳＯＤ、ＧＰＸ的活性明显高于单纯缺血／再灌注时，超微结构损伤亦较轻。因此，说明了含硒Ｓｔ·Ｔｈｏｍａ’ｓ停搏液对大鼠心肌缺血／再灌注的损伤具有保护作用。

We adopted the model of langendorff perfusion in order to observe superoxide dismutase(SOD),Glutathione peroxidase(GPX), Lactate dehydrogenase(LDH), Cretine phosphokinase (CK),malondialdehyde (MDA)and ultrastructure of oxygen free radicals(OFR)cleaning enzymes during ischemia reperfusion of myocardial injury.With time of ischemia prolonging,the contents of LDH,CKand MDA increased,and the activities of SOD and GPX decreased, at the same time, heavy damage of ultrastructure was also observed. But if selenium was added to St. Thoma's cardioplegic solution first,then, ischemia referfusion,the concentration of LDH and CK in the coronary vessel's sinus veflux solution,and the contents of MDA were lower,and the activities of SOD and GPX in mycardical tissue were significantly higher than those in using St.Thoma's Cardioplegic Solution, and the damage of ultrastructure was also slight.This suggests that St. Thoma's Cardioplegic Solution has the protective effect on rats' myocardial ischemia and reperfusion injury.