小苍兰“上农金黄后”球茎组织培养的初步研究

Study on In Vitro Culture of Bulblets from Freesia refracta 'Shangnongjinghuanghou'

以小苍兰优良品种“上农金黄后”的小籽球为外植体,研究了植物生长调节剂对其球茎离体培养及植株再生的影响。结果表明,植物生长调节剂对小苍兰“上农金黄后”小球茎离体培养有着较明显作用,生长素NAA与2,4-D对愈伤组织及不定芽的诱导作用相近,以较低浓度与BA组合诱导效果良好。最佳诱导培养基为MS+BA 2.0mg.L-1(单位下同,略)+NAA 0.2～0.5;最佳生根培养基为MS+NAA 0.2+0.25%活性炭。

Using bulblets of Freesia refracta‘Shangnongjinghuanghou＇ as explants, the influence of plant hormone to its in vitro culture and plant regeneration was studied in this paper. The results showed that plant hormones played an obvious role in tissue culture of the bulblet of Freesia refracta. It＇s better for induction callus and shoot of Freesia refi＇acta using MS basic medium with BA and lower concentration of NAA or 2,4-D. The best medium for induction callus and shoot of Freesia refracta was MS with BA 2.0mg·L^-1（the same unit as below,omission） and NAA 0.2-0.5,and the best medium for rooting was MS with NAA 0.2 and 0.25 % active carbon.