摘要
目的研究制备促肝细胞生长素的新方法。方法以新生小牛肝脏为原料,通过改变酶解、热变性等方法得到促肝细胞生长素成品。结果以胰酶为催化剂制备的促肝细胞生长素,其多肽含量达到了14.97 mg/mL,是文献报道的2倍多。结论在今后的生产中可采用胰酶酶解法制备促肝细胞生长素。
Purpose To prepare hepatocyte promoting growth factor （pHGF） by a new method. Methods Different processes of enzymolysis and thermaldenaturation for pHGF preparation from cattle liver were compared. Results The process using pancreatin as catalyst was the best one. The concentration of polypeptide reached 14.97 mg/mL, 2 times of that of the literatures reported. Conclusion The process using pancreatin as catalyst can be applied in industrialized production of pHGF.
出处
《中国生化药物杂志》
CAS
CSCD
2006年第5期296-298,共3页
Chinese Journal of Biochemical Pharmaceutics
关键词
促肝细胞生长素
制备
提取
hepatocyte promoting growth factor
extraction
preparation
