子宫内膜异位症中COX-2及KGF的表达

Expression of cyclooxygenase2 and keratinocyte growth factor in endometriosis

目的研究环氧化酶-2、角质细胞生长因子在子宫内膜异位症患者卵巢异位组织、在位内膜中的变化,及其与患者临床分期、临床表现及月经周期的关系。方法应用链霉素抗生物素蛋白-过氧化物酶连接免疫组织化学法检测30例子宫内膜异位症卵巢巧克力囊肿患者的卵巢异位组织及在位内膜中角质细胞生长因子、环氧化酶-2的表达。结果环氧化酶-2在正常内膜(53.3%)、子宫内膜异位症在位内膜(70%)及子宫内膜异位症异位组织中(93.3%)的表达阳性率逐渐升高,差异有显著性意义(P<0.01);正常内膜及子宫内膜异位症在位内膜中环氧化酶-2表达受月经周期影响,分泌期的阳性表达率(71.4%,84.6%)高于增生期(37.5%,58.5%),差异有显著性意义(P<0.05,P<0.01);环氧化酶-2的表达与子宫内膜异位症的痛经有关(P<0.05)。角质细胞生长因子在正常内膜(50%)、子宫内膜异位症在位内膜(66.7%)及子宫内膜异位症异位组织中(93.3%)的表达阳性率逐渐升高,差异有显著性意义(P<0.01)。正常内膜及子宫内膜异位症在位内膜中角质细胞生长因子表达受月经周期影响,分泌期的阳性表达率(85.71%,92.3%)高于增生期(18.75%,47.06%),差异有显著性意义(P<0.01,P<0.01),在子宫内膜异位症中环氧化酶-2、角质细胞生长因子二者密切相关(r=0.52)。结论环氧化酶-2、角质细胞生长因子的表达与子宫内膜异位症的发生有关,二者在子宫内膜异位症的发生发展中有协同作用,可能为子宫内膜异位症的治疗提供新的靶点。

Objective To investigate expression of cyclooxygenase2 （COX-2） , keratinocyte growth factor（KGF） in ectopic tissues of ovary, and eutopic endometria of patients with endometriosis （EMs） and their correlation with clinical stages, manifestations of the disease and menstrual cycles of the patients. Methods Using streptomycin avdin cyclooxygenase2 linked immunohistochemistry method, the expression of COX-2 and KGF in ovarian ectopic tissues and eutopic endometria of 30 EMs patients with chocolate cyst （ endometrial cyst ） were detected. Results The positive expression rate of COX-2 in normal endometria and eutopic endometria and ectopic tissues in endometriosis increased more and more（53.3% , 70% , 93.3% , respectively）, and there was significant difference （P 〈0.01 ）. The expression of COX-2 in normal endometria and eutopic endometria in endometriosis was ＇affected by menstrual cycles of the patients： that in secretory phase （71.4% , 84.6% ） was higher than that in proliferative phase （37.5% , 58.5% ） , and there was significant difference（P 〈0. 05, P 〈0.01 ）. While the expression of COX-2 was not associated with clinical stages of endometriosis（ P 〉 0.05 ） , and it was associated with dysmenorrhea in endometriosis （P 〈 0.05 ）. The positive expression rate of KGF in normal endometria and eutopic endometria and ectopic tissues in endometriosis was also increased more and more（50% , 66.7% , 93.3% , respectively） , and there was significant difference （ P 〈 0.01 ）. The expression of KGF in normal endometria and eutopic endometria in endometriosis was also affected by menstrual cycles of the patients： that in secretory phase （85.71% , 92.3% ） was higher than that in proliferative phase （18.75% , 47.06% ）, and there was significant difference（both P 〈 0.01 ）. The expression of KGF was not associated with clinical stages of endometriosis （ P 〉 0.05 ）. In endometriosis, COX-2 and KGF were closely correlated with each other（ r = 0.52）. Conclusion COX-2 and KGF may play important roles in development of endometriosis, and they have close relation in occurrence and development of endometriosis and this may provide new treatment targets for endometriosis.