CD_（44）基因变异性表达与膀胱癌的关系

RelationshipbetwentheabnormalexpressionofCD44geneandbladdercancer

为探讨膀胱癌的发生与ＣＤ４４基因变异表达之间的相关性，作者采用逆转录－聚合酶链反应和印迹杂交技术对４２例膀胱组织标本（２０例膀胱癌及其１０例正常膀胱组织，８例良性前列腺增生患者的正常膀胱组织和４例膀胱炎性组织）中ＣＤ４４基因含Ｖ６外显子的变异表达产物进行检测。结果显示：所有膀胱癌组织标本中均检测到ＣＤ４４基因含Ｖ６外显子的异常拼接变异转录物的过量表达，而２２例非癌膀胱组织中均未检测到这种异常的拼接变异转录物，且２例转移性膀胱癌与１８例非转移癌组织中上述变异表达产物的量及表现形式有差别。提示ＣＤ４４基因的变异表达（异常拼接和过量表达）与膀胱癌的恶性表型和转移特性密切相关，这种变异表达产物有可能成为膀胱癌早期诊断和对其转移潜能进行早期检测的较为理想的分子标记物，值得进一步研究。

Reversetranscription-polymerasechainreaction(RT-PCR)andSouthernblothybridiza-tionwereusedtodetectexonV6-containingisoformsofCD44genein42surgicalbladderspecimens(20casesofbladdercancer,10casesofcorrespondingnormalbladdertissue,8non-neoplasticbladdertissuefromthebenignprostatichyperplasia,and4cystitis).Alsamplesofbladdercancertisueshowedover-expresionofmanyabnormalalternatively-splicedproductscontainingtranscriptsfromexonV6oftheCD44geneexceptthosefrom22non-neoplastictissue.Furthermore,thebandpaternpermiteddiferen-tiationbetwenthe2casesofmetastaticbladdertumorsandthe18casesofnonmetastatictissue.OurresultssuggestthatthereisaremarkablerelationshipbetweentheabnormalCD44geneexpresion(in-appropriatesplicingandoverexpresion)andmalignantphenotypeandmatastasisofbladdercancer.TheCD44splicevariantsmayprovetobeamoreidealtumormarkerfortheearlydiagnosisofbladdercancerandfortheearlydetectionofmetastaticpotentialinsurgicalbiopsyspecimens,whichdeservesfurthere-valuation.