金属硫蛋白在肝细胞癌中的表达及其临床意义

The Expression of MTs in Hepatocellular Carcinoma and Its Clinical Significance

目的研究MTs在HCC中的表达及其与临床病理特征的关系。方法选取48例HCC手术切除的新鲜标本和10例正常肝组织,10%福尔马林固定,常规石蜡包埋,每个标本行连续切片,厚4μm。应用SABC免疫组化法,以MTs单克隆抗体对HCC组织切片进行染色。用χ2检验、方差分析、t检验和Fisher确切概率法,对MTs在HCC中的表达及其与临床病理特征的关系进行研究。结果MTs在HCC癌组织中的阳性率(60.42%)明显低于癌旁及正常肝组织(P<0.05);MTs在高分化、中分化和低分化HCC中的评分分别是3.00±1.17,2.36±1.65,1.85±1.76,高分化明显高于低分化(P<0.05);它与其它病理特征无显著关系。结论MTs在HCC癌中的表达明显降低,且HCC病理分化差者,表达明显下降,提示MTs可能抑制肝癌的发生和发展。

Objective To study the expression of MTs in HCC and the relationship between the expression and clinical pathological characterization. Methods 48 fresh resected specimens from the patients with HCC and 10 normal liver tissue were selected, fixed with 10% formalin, paraffin embed in routine, each sample were proceed serial section, about 4 μn thick, tissue section of HCC were stained by SABC immunohistochemistry, with MTs in HCC and the relationship between the expression and clinical pathological characterization were studied with X^2 check, t check and Fisher precise probability. Results Positive rates of MTs in the tissue of HCC were 60.42%, it was obviously lower than paracarcinoma tissue and normal liver tissue（ P 〈 0.05） ; The scores of MTs in well middle and poorly differentiated HCC were 3.00 ± 1.17 2.36 ± 1.65 and 1.85 ± 1.76, respectively. The scores of MTs in well - differentiated HCC were significantly higher than those in poorly - differentiated cancer（ P 〈 0. 05 ） ; The expression of MTS was not associated with other pathological characterization. Conclusion The expression of MTs in the tissue of HCC was obviously lower than paracarcinoma tissue and normal liver tissue, and it was associated with differentiation degree of HCC. It showed that MTS restrained possibly the occurrence and develop- ment of HCC.