小肠癌DNA倍体定量分析及其临床意义的研究

DNAquantitativeanalysisofsmalbowlcarcinomaanditsclinicalsignificance

作者应用计算机图像分析ＤＮＡ定量的方法对２８例小肠癌、５例小肠腺瘤、６例正常小肠组织中的异倍体所占的比例及ＤＮＡ含量的１２个参数进行了检测。结果表明：小肠癌中的异倍体数量明显高于小肠腺瘤，其中ＤＮＡ熵值（ＥＤ）、２倍体偏移指数（２ＣＤＩ）、ＤＮＡ恶性分级（ＤＧＮ）、５倍体超过率（５ＣＥＲ）、９倍体超过率（９ＣＥＲ）、２倍体偏移熵数（ＤＤＱ）、平均倍体（ＭＰ）七个ＤＮＡ参数对判断小肠良、恶性病变有重要意义；ＤＮＡ含量与小肠癌的临床病理组织分化程度无关，但异倍体小肠癌较非异倍体小肠癌恶性度高，易发生浸润和转移，术后生存率低，预后较差。ＤＮＡ参数中的ＤＮＡ指数（ＤＩ）、众数值（ＭＶ）、ＤＮＡ恶性分级（ＤＧＮ）、干系倍体值（ＳＰ）、９倍体超过率（９ＣＥＲ）五个参数与小肠癌的预后关系密切。

Byusingcomputercytophotometricanalysis,aneuploidaswelasother12parametersoftissueDNAcontentweremeasuredin28casesofsmalbonlcancer,5casesofintestinaladenoma,6casesofnormalintestinaltisues.Thenumberofaneuploidtumorsinintestinalcancerwasmuchhigherthaninintestinaladenoma.7DNAparameters(EntropyofDNA,2CDI,DNA-gradeofneoplasia,5CExceedingRate,9CExcedingRate,DiploidDeviationQuotientandMeanPloidy)werethevaluablein-dexesthatcoulddeterminethequalityofintestinallesions,benignormalignant.DNAcontentdidn′tcorelatewiththeclinicalydiferentiateddegreeofpathologicaltissues,butaneuploidintestinalcancershadahighermalignantdegreethannon-aneuploidones,andweremoreliabletoinvadeandmetastasizewithlowpostoperativesurvival-rateandpoorprognosis.5DNAparameters(DNA-Index,MedalVal-ue,DNA-gradeofneoplasia,StemlinePloidand9CExcedingRate)hadcloserelationtotheprognosisofintestinalcancer,possesingimportantvaluetotheprognosticanalysisofintestinalcancer.