肝硬变大鼠行部分肝叶切除术后枯否细胞DNA和RNA合成功能的变化

ChangesofKupffercellDNAandRNAsyntheticfunctionsafterpartialbepatectomyincirrboticrats

作者通过肝硬变大鼠模型，行部分肝叶切除术，动态观察再生肝脏Ｋｕｐｆｆｅｒ细胞ＤＮＡ和ＲＮＡ合成功能变化规律，探讨肝再生机制与肝细胞功能衰竭的关系。７０只健康的雄性Ｗｉｓｔａｒ大鼠，体重２２０－２５０ｇ，分为肝硬变大鼠部分肝叶切除术组（Ｃ－ＰＨ）．正常大鼠部分肝叶除术组（Ｎ－ＰＨ）和假手术组（ＳＯ）。Ｎ－ＰＨ组和Ｃ－ＰＨ组均切除大鼠肝脏的左叶和中叶。各组于不同时相用流式细胞仪测定Ｋｕｐｆｆｅｒ细胞的ＤＮＡ和ＲＮＡ含量。结果表明：Ｃ－ＰＨ组枯否氏细胞Ｇ２期和Ｍ期ＤＮＡ合成的高峰是在术后２４小时以后，明显落后于Ｎ－ＰＨ组；其Ｋｕｐｆｆｅｒ细胞增殖系数的峰值在术后４８小时，以后持续高于Ｎ－ＰＨ组和ＳＯ组，但其残肝的重量在术后１周时低于Ｎ－ＰＨ组；Ｋｕｐｆｆｅｒ细胞ＲＮＡ含量一直低于Ｎ－ＰＨ组和ＳＯ组，于术后一周时其ＲＮＡ合成的水平才恢复到术前的水平。作者认为，肝硬变大鼠行肝叶部分切除术后枯否氏细胞功能受抑制，其启动时间的推迟和再生周期的延长是造成术后肝细胞再生能力低下和肝细胞功能衰竭的一个重要原因。

WestudiedthechangesofDNAandRNAsyntheticfunctionsofregeneratinglivercellsafterpartialhepatectomyincirrhoticratsanddiscussedtherelationshipbetweenliverregenerationandliverfunctionalfailure.SeventyhealthyWistarratsofbothsexes,weighing200-250g,wererandomizedintosham-operated(SO),partialhepatectomyincirrhoticrats(C-PH)andpartialhepatectomyinnor-malrats(N-PH).Theleftandmiddlelobesweredissected.ThesyntheticDNApeakofKupffercells(KC)intheG2andMphaseinC-PHwasfoundafter24hrofoperation.TheregeneratingindexpeakofKCwasat48hrafteroperation,whichwashigherthantheothergroups,buttheliverweightsinC-PHwaslowerthanthatintheN-PHat1weekafteroperation.TheRNAcontentofKCmarkedlydecreasedandreturnedtonormallevelsat1weekafteroperation.WethinkthatKCfunctionsaresuppressedafterpartialhepatectomyincirrhoticrats.Itisanimportantcauseofhepatocyteregenerationfunctionde-creaseandliverfunctionalfailurefordelayoflivercellregneration'start'andtheregenerationcellcy-cle.