橄榄叶黄酮类抗氧化物质的结构分析研究

Study on the Analysing Structure of Flavonoids Antioxidants from Olive Leave

通过超声强化提取法提取橄榄叶黄酮,采用聚酰胺柱层析和制备薄层层析对橄榄叶黄酮提取液进行分离纯化,通过抗氧化活性比较,筛选抗氧化活性最好的样品C3,运用高效液相色谱法(HPLC)、紫外光谱法(UV)、红外光谱法(IR)、液质联用法(LC-MS)、核磁共振法(1HNMR,13CNMR),对样品C3进行结构分析,确定为木犀草素。

The flavonoids of olive leaves was extractied by ultrasonic enhanced extraction, then separated and purified with polyamide column chromatography and PTLC. Sample C3 was selected by screen the antioxidative activity and its structure was elucidated by UV, IR, LC-MS, ^1HNMR, ^13CNMR, and was identified as Luteolin.