黄芩苷对肺炎衣原体感染小鼠血清sICAM-1、sVCAM-1及E选择素的调节作用

The Influence of Baicalin on the Level of Serum sICAM-1,sVCAM-1 and sE-slectin in CPn-Infected Mice

目的探讨肺炎衣原体(CPn)感染高脂饮食小鼠后血清细胞间黏附分子-1(sICAM-1)、血管细胞黏附分子-1(sVCAM-1)、E选择素(sE-slectin)的变化及黄芩苷调节作用。方法将50只小鼠随机分为黄芩苷高剂量组、黄芩苷低剂量组、阿奇霉素组、模型组、正常对照组。除正常对照组外均于喂饲高脂饲料1周后,经一侧鼻腔吸入含CPn的培养液,每周1次,共3次。最后1次接种5d后开始给药至实验结束,第1次接种后第18周处死全部动物。结果模型组血清sICAM-1、sVCAM-1、sE-slectin均较正常对照组升高(P<0.01);与模型组比较,黄芩苷高剂量组、低剂量组和阿奇霉素组3个指标均有不同程度下降,其中黄芩苷低剂量组和阿奇霉素组sICAM-1和sE-slectin显著降低(P<0.01)。结论CPn感染高脂饮食小鼠可使血清sICAM-1、sVCAM-1、sE-slectin水平升高,黄芩苷高剂量、低剂量和阿奇霉素均具有不同程度的调节作用,从而在一定程度上抑制动脉粥样硬化的形成。

Objective To study the variation of serum slCAM- 1, sVCAM- 1 and sE - slectin in diet - induced hyperlipidemic and chronic CPn - infected mice and the intervention of haicalin. Methods Eight - week - old SPF male C57BL/6J mice were divided into 5 groups randomly： High- dosagehaicalin group （group Ⅰ ）, low - dosage - haicalin group （group Ⅱ ）, azithromycin group（group Ⅲ ）, model group （group Ⅳ ） and normal group （group Ⅴ ）. Following cholesterol- supplemented diet for one week, group Ⅰ , group Ⅱ , group Ⅲ , group Ⅳ and group Ⅴ were inoculated with CPn at （0.5 × 10^7 - 1.0 ×10^7） IFU per inoculation. Each mouse in these groups was inoculated once a week for 3 consecutive weeks. Administration started 5 days after the last inoculation. Results The level of serum slCAM - 1, sVCAM- 1 and sE - slectin increased in group Ⅳ. All of them in three treatment groups declined at different levels. Conclusion CPn infection could raise the level of serum slCAM - 1, sVCAM- 1 and sE- slectin in di- et- induced hyperlipidemic C57BL/6J mice. Early treatment by baicalin or azithromycin could lower them in some extent.