蒲黄提取物对汞损伤SD大鼠原代培养神经细胞抗氧化能力的影响

Effect of Pollen Typhae Extracts on Antioxidant Activity of Mercury-injuried Primary Cultured Neurons

目的观察氯化汞损伤神经细胞后抗氧化指标的变化及蒲黄提取物对受损神经细胞的修复作用。方法大鼠神经细胞用浓度为0.2mmol·L-1氯化汞损伤24h,检测细胞中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果损伤后的大鼠神经细胞中GSH-Px和SOD活性显著下降,MDA含量显著上升。神经细胞突触显著减少,胞间质脱落,变形。经蒲黄水提物和醇提物共同培养后,大鼠神经细胞中GSH-Px活性和SOD活性上升,MDA含量下降。神经细胞突触显著增加,胞间质完好,形态趋于正常。结论蒲黄提取物无论是水提物还是醇提物均可显著提高汞损伤神经细胞的抗氧化能力,能促进损伤细胞的恢复,对神经细胞具有保护作用。

Aim To study changes of certain antioxidant indexes and the efficacy of pollen typhae extract against mercury chloride-induced lipid peroxidation in cultured hippoeampal neurons. Methods After 24 hours of exposure to 0.2mmol·L^-1 mercuric chloride, GSH-Px and SOD activities and MDA content were measured. Results In the injured neurons, the activities of GSH-Px and SOD decreased and the content of MDA increased significantly, while neuron synaptics shrunk and cytoplasm distorted remarkably in morphology. In the culture containing pollen typhae water and ethanolic extracts, the activity of GSH-Px and SOD in injured nerones increased, and MDA content decreased; both the synaptic and cytoplasm morphology tended to be normalized. Conclusion The results indicated that both water and ethanolic extract of pollen typhae significantly improved the antioxidant activity and promoted the recovery of mercury-injured neurons, promising effective protection for the mercury-injured neurons.