高效液相色谱法测定枳实导滞丸中橙皮苷和黄芩苷含量

Determination of Hesperidin and Baicalin In Zhishidaozhi Wan by HPLC

目的 建立HPLC测定枳实导滞丸中橙皮苷和黄芩苷含量的方法。方法 Intersil C18分析色谱柱（4．6mm ID×250mm，粒径5μm），C18保护柱（4．6mm×5mm，5μm）。流动相：乙腈-水（20：80，含0．7％三乙胺，磷酸调pH值2．5），流速1．0ml／min，检测波长276nm。结果 橙皮苷、黄芩苷保留时间分别为8．5、15．6min，与各自相邻峰的分离度〉1．5。以峰面积Y对进样量X（μg）线性回归，橙皮苷回归方程：Y=20367313．7X＋3946464．7，r=0．9996，线性范围0．480-2．880μg。黄芩苷回归方程：Y=63795699．4X＋6084871．0，r=0．9996，线性范围0．360～2．160μg。橙皮苷、黄芩苷回收率为101．3％，99．5％；RSD为1．6％，1．5％（n=9）。结论 本方法操作简便，结果准确可靠，可用于枳实导滞丸中橙皮苷和黄芩苷的含量测定。

Aim To develop an HPLC quantitative method for the determination of Hesperidin and Baicalin in Zhishidaozhi Wan. Methods The chromatographic conditions include column Intersil C18, mobile phase： acetonitrile- water（20： 80, which contains 0.7 % triethylamine that was adjusted to pH 2.5 by phosphoric acid） . The flow rate was 1.0 ml/min and monitored at 276 nm. Results the retention time of Hesperidin and Baicalin as 8.5 min and 15. 6 min respectively. The number of theoretical plates calculated by Hesperidin and Baicalin peak as 2 500 and 2 600 respectively, The regress equation for Hesperidin was Y = 20 367 313.7X ＋3 946 464.7, r = 0.999 6,and the linear range was 0.480-2.880μg. The regress equation for Baicalin was Y = 63 795 699.4X ＋ 6 084 871.0, r=0.999 6, and the linear range was 0.360-2. 160μg. The average recovery of Hesperidin and Baicalin were 101.3%, RSD 1.6% （ n =9） % and 99.5%, RSD 1.5% （ n =9） % respectively. Conclusion The method was sensitive, time-sav ing and accurate for the determination of Hesperidin and Baicalin In Zhishidaozhi Wan .